Part:BBa_K404107:Design
Beta-Globin-Intron
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
For designing the BioBrick part, the iGEM team Freiburg used primers in the RFC10 standard and performed a PCR. The PCR product was digested using XbaI and PstI followed by ligation into the iGEM standard plasmid pSB1C3.
Source
The plasmid was PCR amplified using the vector pAAV_MCS as DNA template.
References
Nott, A., Le Hir, H., & Moore, M. J. (2004). Splicing enhances translation in mammalian cells: an additional function of the exon junction complex. Genes & development, 18(2), 210-22. doi: 10.1101/gad.1163204.
Nott, A., Meislin, S. H., & Moore, M. J. (2003). A quantitative analysis of intron effects on mammalian gene expression. RNA (New York, N.Y.), 9(5), 607-17. doi: 10.1261/rna.5250403.et.
Valencia, P., Dias, A. P., & Reed, R. (2008). Splicing promotes rapid and efficient mRNA export in mammalian cells. Proceedings of the National Academy of Sciences of the United States of America, 105(9), 3386-91. doi: 10.1073/pnas.0800250105.